Kisspeptin-10 (10 mg Vial) Dosage Protocol
Kisspeptin-10 is the 10-amino acid bioactive C-terminal fragment of kisspeptin-54 (metastin). Kisspeptin neurons in the hypothalamus provide the key upstream trigger for GnRH pulsatile release — acting as a 'master switch' for the HPG axis. Research focuses on LH pulsatility, reproductive endocrinology, and sexual function.
⚡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous or IV (research settings) | Frequency: Pulsatile or intermittent
| Application | Dose | Route | Notes |
|---|---|---|---|
| LH pulse research | 1–4 nmol/kg | IV (research) | Standard pharmacokinetic study dose |
| Sub-Q research | 1–3 mg | Sub-Q | Longer absorption; less acute LH peak |
| Pulsatile protocol | 1 mg every 90 min | Sub-Q/IV | Mimics natural kisspeptin pulsatility |
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the vial; avoid foaming.
- Gently swirl until dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Planning
| Item | Research Use (per study) |
|---|---|
| Kisspeptin-10 vials (10 mg) | Varies by protocol |
| IV/Sub-Q syringes | Per injection frequency |
| Bacteriostatic water (10 mL) | 1 per 2 vials |
Mechanism of Action
Kisspeptin is a neuropeptide encoded by the KISS1 gene, cleaved into bioactive forms of varying lengths (kisspeptin-54, -14, -13, -10). Kisspeptin-10 is the most potent and commonly studied fragment. Kisspeptin neurons (primarily in the arcuate nucleus and anteroventral periventricular nucleus) project to GnRH neurons and express GPR54 (Kiss1R) receptors. Kisspeptin binding to GPR54 triggers action potential generation in GnRH neurons, causing GnRH pulse generation and downstream LH and FSH release.
This makes kisspeptin the upstream regulator of GnRH pulsatility — understanding kisspeptin signaling is fundamental to reproductive neuroendocrinology. GPR54 loss-of-function mutations cause hypogonadotropic hypogonadism in humans; GPR54 agonism restores LH pulsatility. The research applications range from fertility to puberty timing to sexual function.
Research Findings & Safety Profile
- GPR54 activation by kisspeptin-10 robustly stimulates LH release in both sexes in human studies.
- Kisspeptin infusion restores LH pulsatility in patients with hypogonadotropic hypogonadism.
- Enhances sexual arousal and attraction processing in fMRI studies at Imperial College London.
- Loss-of-function GPR54 mutations cause complete failure of puberty and hypogonadism — validating the pathway.
- Possible effects: LH surge (desired), mild GI effects at high doses, flushing.
- Primarily a research tool for HPG axis pharmacology; limited therapeutic protocols established.
Storage
| State | Temperature | Duration | Notes |
|---|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months | Dry, dark conditions |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days | Avoid freeze-thaw; protect from light |