🧬 Longevity & Bioregulator
Epitalon / Epithalon (10 mg Vial) Dosage Protocol
Epitalon (Ala-Glu-Asp-Gly) is a synthetic tetrapeptide developed by Vladimir Khavinson at the St. Petersburg Institute of Bioregulation. Research focuses on its effects on telomerase activation, pineal gland regulation (melatonin), and longevity biomarkers in multiple model systems including humans.
âš¡ Quickstart Highlights
Reconstitution
2.0 mL BAC water → 5.0 mg/mL
Daily Range
5–10 mg/day during cycle
1 U-100 Unit =
50 mcg (0.05 mg)
Cycle Structure
10–20 day cycles, 2–3× per year
Dosing & Reconstitution Guide
Route: Subcutaneous or IV | Frequency: Once or twice daily during active cycle
| Phase | Daily Dose | U-100 Units | Volume (mL) | Duration |
|---|---|---|---|---|
| Standard cycle | 5 mg/day | 100 units | 1.0 mL | 10 days, 2–3×/year |
| Extended cycle | 10 mg/day | 200 units | 2.0 mL (split) | 20 days, 1–2×/year |
Epitalon is typically administered in cycles rather than continuously. The classic Khavinson protocol uses 10-day courses, 2–3 times per year (spring/autumn or quarterly). Some researchers extend to 20-day courses. Split large daily doses (≥10 mg) across two injections.
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water.
- Inject slowly down the vial wall.
- Gently swirl until dissolved.
- Refrigerate at 2–8°C; use within current cycle.
Supplies Planning
| Item | 10-Day Cycle (5 mg/day) | 20-Day Cycle (10 mg/day) |
|---|---|---|
| Epitalon vials (10 mg each) | 1 vial | 2 vials |
| Insulin syringes (U-100, 1 mL) | 10 | 20–40 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 1–2 × 10 mL |
| Alcohol swabs | 1 × 100-pack (shared) | 1 × 100-pack |
Mechanism of Action
Epitalon (Ala-Glu-Asp-Gly) was developed by Professor Vladimir Khavinson at the St. Petersburg Institute of Bioregulation and Gerontology. It is hypothesized to be a bioregulator peptide derived from the pineal gland extract (Epithalamin).
Key proposed mechanisms under study:
- Telomerase activation: Epitalon has been reported to activate telomerase enzyme in human somatic cells in vitro, potentially extending telomere length — a key biomarker of cellular aging.
- Pineal regulation: Appears to stimulate melatonin production and normalize circadian rhythm biomarkers, with downstream effects on antioxidant status and immune function.
- Antioxidant effects: Reduces oxidative stress markers in multiple model systems.
- Neuroendocrine normalization: Multiple human studies (primarily Khavinson's group) report improvements in neuroendocrine biomarkers in elderly populations with cyclic Epitalon administration.
Research Findings & Safety Profile
- Human studies: Khavinson et al. reported improvements in melatonin secretion, antioxidant status, and life expectancy biomarkers in elderly populations over 3–15 year follow-up.
- In vitro: Activation of telomerase in human fetal fibroblasts and retinal pigment epithelial cells.
- Animal models: Extended lifespan in mice and fruit flies in multiple studies.
- Well-tolerated in human studies; no significant adverse events reported at studied doses.
- Research base primarily from Russian academic institutions; limited independent replication in Western literature.
- Pineal/melatonin pathway suggests potential benefits for sleep quality and circadian regulation.
Storage
| State | Temperature | Duration | Notes |
|---|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months | Dry, dark conditions |
| Reconstituted | 2–8°C (35–46°F) | Duration of cycle only | Prepare fresh per cycle |
âš Research Use Only: Epitalon is an investigational compound. Research base is primarily from a single Russian academic group (Khavinson et al.); independent replication remains limited. Cyclic protocols are recommended rather than continuous use.
References
1
Khavinson VK et al. 'Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells' — Bull Exp Biol Med, 2003 View source ↗
2
Kossoy G et al. 'Effect of epithalon on the age-associated changes and T-cell differentiation factor in lymphocytes in culture of epithelial cells of human fetal thymus' — Neuro Endocrinol Lett, 2003
3
Anisimov VN et al. 'Effect of Epitalon on biomarkers of aging, life span and spontaneous tumor incidence in female Swiss-derived SHR mice' — Biogerontology, 2003 View source ↗