DSIP / Delta Sleep-Inducing Peptide (10 mg Vial) Dosage Protocol
DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) isolated from rabbit cerebral venous blood. It is studied for its sleep-modulating, anti-stress, neuroendocrine-normalizing, and antioxidant properties in multiple model systems.
âš¡ Quickstart Highlights
Dosing & Reconstitution Guide
Route: Subcutaneous | Frequency: Once daily before sleep (short cycles)
| Phase | Dose | U-100 Units | Volume | Duration |
|---|---|---|---|---|
| Standard cycle | 250–500 mcg | 5–10 units | 0.05–0.10 mL | 5–10 days |
| Conservative | 250 mcg | 5 units | 0.05 mL | 5 days; repeat as needed |
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water into a sterile syringe.
- Inject slowly down the inside glass wall of the vial; avoid foaming.
- Gently swirl until dissolved. Do not shake.
- Label with reconstitution date. Refrigerate at 2–8°C; use within 28 days.
Supplies Planning
| Item | 10-Day Cycle | 3 Cycles |
|---|---|---|
| DSIP vials (10 mg) | 1 vial | 3 vials |
| Insulin syringes (30-unit) | 10 | 30 |
| Bacteriostatic water (10 mL) | 1 × 10 mL | 2 × 10 mL |
| Alcohol swabs | shared | 1 × 100-pack |
Mechanism of Action
DSIP was originally isolated from rabbit cerebral venous blood in 1974 by Monnier et al. after identifying that venous blood from sleeping rabbits could induce slow-wave (delta) sleep when infused into awake rabbits. It is a nonapeptide that crosses the blood-brain barrier through a specific transport mechanism.
Proposed mechanisms include: modulation of delta-wave sleep (deep restorative slow-wave sleep) via direct CNS action; reduction of basal corticotropin (ACTH) levels and normalization of stress-reactive HPA axis responses; interaction with opioid receptors contributing to anti-stress effects; antioxidant activity; and regulation of GH pulsatility during sleep. Human studies show improvements in sleep architecture, reduced sleep onset latency, and normalization of disturbed circadian rhythms.
Research Findings & Safety Profile
- Originally isolated 1974 from cerebral venous blood of sleeping rabbits; can induce delta-wave sleep when transfused.
- Human studies: improvements in slow-wave sleep, sleep onset latency, and subjective sleep quality.
- Anti-stress effects: reduces ACTH elevation in stress models; normalizes HPA axis reactivity.
- Antioxidant properties demonstrated in various oxidative stress models.
- Short cycles (5–10 days) are standard; research base smaller than for approved sleep medications.
- Well-tolerated in studied doses; adverse effect profile not well established due to limited clinical data.
Storage
| State | Temperature | Duration | Notes |
|---|---|---|---|
| Lyophilized | −20°C (−4°F) | Up to 24 months | Dry, dark conditions |
| Reconstituted | 2–8°C (35–46°F) | Up to 28 days | Avoid freeze-thaw; protect from light |